Cell-cycle-dependent expression of DNA primase activity
نویسندگان
چکیده
منابع مشابه
Cell cycle-dependent regulation of human DNA polymerase alpha-primase activity by phosphorylation.
DNA polymerase alpha-primase is known to be phosphorylated in human and yeast cells in a cell cycle-dependent manner on the p180 and p68 subunits. Here we show that phosphorylation of purified human DNA polymerase alpha-primase by purified cyclin A/cdk2 in vitro reduced its ability to initiate simian virus 40 (SV40) DNA replication in vitro, while phosphorylation by cyclin E/cdk2 stimulated its...
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A primase activity which permits DNA synthesis by yeast DNA polymerase I on a single-stranded circular phi X174 or M13 DNA or on poly(dT)n has been extensively purified by fractionation of a yeast enzyme extract which supports in vitro replication of the yeast 2-microns plasmid DNA (Kojo, H., Greenberg, B. D., and Sugino, A. (1981) Proc. Natl. Acad. Sci. U.S.A. 78, 7261-7265). Most of this DNA ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1987
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1987.tb10992.x